Genetic diversity and seroprevalence of Toxoplasma gondii in COVID19 patients; a first case-control study in Iran.
BMC Infect Dis
; 24(1): 42, 2024 Jan 03.
Article
in En
| MEDLINE
| ID: mdl-38172676
ABSTRACT
BACKGROUND:
Toxoplasmosis is a serious or life-threatening disease in immunosuppressed patients and pregnant women. This study examined the likely association between Toxoplasma gondii infection and COVID-19 patients with moderate illness.METHODS:
Seventy blood samples were collected from patients at the Health Reference Laboratory of Tabriz, Northwest Iran from April 2021 to September 2021. In addition, 70 healthy subjects of the same age (37 ± 15 years) and sex distribution were ethnically matched. Sera samples were examined for the detection of anti-Toxoplasma antibodies using ELISA. Nested-PCR targets were amplified based on the B1 and GRA6 genes. GRA6 amplicons were subjected to sequencing and phylogenetic analysis.RESULTS:
The seroprevalence of toxoplasmosis based on IgG titer was 35.7% in the COVID19 patients and 27.1% in the control group, representing not to be associated with the Toxoplasma seropositivity in COVID19 patients (P = 0.18) compared to healthy subjects. Anti-T. gondii IgM was not found in any of the patients and healthy individuals. According to PCR amplification of the B1 and GRA6 genes, the frequency of T. gondii in COVID-19 patients was 14.2% (10/70). However, no T. gondii infection was detected in the healthy group. The CD4+T cell count was relatively lower in toxoplasmosis-infected patients (430-450 cells/mm3) than in control group (500-1500 cells/mm3). High genetic diversity (Hd 0.710) of the type I strain of T. gondii was characterized in the patients. Present results showed that consumption of raw vegetables and close contact with stray cats can increase the transmission of T. gondii to COVID-19 patients (P < 0.01).CONCLUSIONS:
The current study revealed that T. gondii type I infection is unequivocally circulating among the COVID-19 patients in Tabriz; However, no significant association was observed between the occurrence of Toxoplasma and the severity of COVID-19. To make more accurate health decisions, multicenter investigations with a larger sample size of different ethnic groups of the Iranian population are needed.Key words
Full text:
1
Collection:
01-internacional
Health context:
1_ASSA2030
/
2_ODS3
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3_ND
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4_TD
Database:
MEDLINE
Main subject:
Toxoplasma
/
Toxoplasmosis
/
COVID-19
Type of study:
Clinical_trials
/
Diagnostic_studies
/
Etiology_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
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Animals
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Female
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Humans
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Middle aged
/
Pregnancy
Country/Region as subject:
Asia
Language:
En
Journal:
BMC Infect Dis
Year:
2024
Document type:
Article