A tool for live-cell confocal imaging of temperature-dependent organelle dynamics.
Microscopy (Oxf)
; 73(4): 343-348, 2024 Jul 30.
Article
in En
| MEDLINE
| ID: mdl-38217102
ABSTRACT
Intracellular organelles alter their morphology in response to ambient conditions such as temperature to optimize physiological activities in cells. Observing organelle dynamics at various temperatures deepens our understanding of cellular responses to the environment. Confocal laser microscopy is a powerful tool for live-cell imaging of fluorescently labeled organelles. However, the large contact area between the specimen and the ambient air on the microscope stage makes it difficult to maintain accurate cellular temperatures. Here, we present a method for precisely controlling cellular temperatures using a custom-made adaptor that can be installed on a commercially available temperature-controlled microscope stage. Using this adaptor, we observed temperature-dependent organelle dynamics in living plant cells; morphological changes in chloroplasts and peroxisomes were temperature dependent. This newly developed adaptor can be easily placed on a temperature-controlled stage to capture intracellular responses to temperature at unprecedentedly high resolution.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Temperature
/
Organelles
/
Chloroplasts
/
Microscopy, Confocal
Language:
En
Journal:
Microscopy (Oxf)
Year:
2024
Document type:
Article