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An Engineered Biarylitide Cross-Linking P450 from RiPP Biosynthesis Generates Alternative Cyclic Peptides.
Treisman, Maxine; Coe, Laura; Zhao, Yongwei; Sasi, Vishnu Mini; Gullick, Jemma; Hansen, Mathias H; Ly, Aviva; Leichthammer, Victor; Hess, Caroline; Machell, Daniel L; Schittenhelm, Ralf B; Hooper, Joel; Jackson, Colin J; Tailhades, Julien; De Voss, James J; Cryle, Max J.
Affiliation
  • Treisman M; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.
  • Coe L; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
  • Zhao Y; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Sasi VM; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.
  • Gullick J; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
  • Hansen MH; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
  • Ly A; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Leichthammer V; Research School of Chemistry, The Australian National University, Acton, ACT 2601, Australia.
  • Hess C; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.
  • Machell DL; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
  • Schittenhelm RB; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.
  • Hooper J; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
  • Jackson CJ; Department of Chemistry, Monash University, Clayton, VIC 3800, Australia.
  • Tailhades J; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.
  • De Voss JJ; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Clayton, VIC 3800, Australia.
  • Cryle MJ; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, EMBL Australia, Clayton, VIC 3800, Australia.
Org Lett ; 26(9): 1828-1833, 2024 Mar 08.
Article in En | MEDLINE | ID: mdl-38417822
ABSTRACT
Cytochrome-P450-mediated cross-linking of ribosomally encoded peptides (RiPPs) is rapidly expanding and displays great potential for biocatalysis. Here, we demonstrate that active site engineering of the biarylitide cross-linking enzyme P450Blt enables the formation of His-X-Tyr and Tyr-X-Tyr cross-linked peptides, thus showing how such P450s can be further exploited to produce alternate cyclic tripeptides with controlled cross-linking states.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Peptides, Cyclic Language: En Journal: Org Lett Year: 2024 Document type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Peptides, Cyclic Language: En Journal: Org Lett Year: 2024 Document type: Article