Progress, Challenges and Opportunities of NMR and XL-MS for Cellular Structural Biology.
JACS Au
; 4(2): 369-383, 2024 Feb 26.
Article
in En
| MEDLINE
| ID: mdl-38425916
ABSTRACT
The validity of protein structures and interactions, whether determined under ideal laboratory conditions or predicted by AI tools such as Alphafold2, to precisely reflect those found in living cells remains to be examined. Moreover, understanding the changes in protein structures and interactions in response to stimuli within living cells, under both normal and disease conditions, is key to grasping proteins' functionality and cellular processes. Nevertheless, achieving high-resolution identification of these protein structures and interactions within living cells presents a technical challenge. In this Perspective, we summarize the recent advancements in in-cell nuclear magnetic resonance (NMR) and in vivo cross-linking mass spectrometry (XL-MS) for studying protein structures and interactions within a cellular context. Additionally, we discuss the challenges, opportunities, and potential benefits of integrating in-cell NMR and in vivo XL-MS in future research to offer an exhaustive approach to studying proteins in their natural habitat.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
JACS Au
Year:
2024
Document type:
Article