Comparative evaluation of antifungal activity of Sodium Hypochlorite, Calcium Hypochlorite and modified Salt Solution associated with passive ultrasonic irrigation against Candida albicans - An In-Vitro study.

ABSTRACT

Aim: The study evaluated the antifungal activity of sodium hypochlorite (NaOCl), calcium hypochlorite (Ca(OCl)2), and modified salt solution (MSS) assisted with passive ultrasonic irrigation against Candida albicans. Materials and Methods: One hundred and thirty-six single-rooted premolars were decoronated and enlarged up to a file #45, autoclaved, inoculated with C. albicans, and incubated for 72 h. The samples were randomly distributed into eight groups (n = 17) according to the protocol for decontamination G1 No treatment, G2 Distilled water (DW), G3 2.5% NaOCl, G4 2.5% NaOCl + ultrasonic activation (US), G5 2.5% Ca(OCl)2, G6 2.5% Ca(OCl)2 + US, G7 MSS, G8 MSS + US. Microbiological testing (Colony forming Unit [CFU] counting) was performed before and after the treatment. Statistical Analysis: Data were subjected to the one-way analysis of variance followed by the Tukey's post hoc test (P < 0.05). Results and Conclusion: Groups 1 and 2 showed the highest mean contamination (5.41 and 4.31 log10 CFU/mL, respectively), which was statistically different from all the other groups (P < 0.001). G4 showed the lowest mean contamination (0.24 log10 CFU/mL) with statistically significant value (P < 0.001). 2.5% NaOCl with ultrasonic activation can aid in significant fungal reduction. Ultrasonic activation of 2.5% NaOCl, 2.5% Ca(OCl)2, and MSS was also found to have improved antifungal activity against C. albicans.