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Determination of carbamazepine in fingerprints: a feasibility study to evaluate adherence in epilepsy patients.
Müller, Isadora Ritter; Grandi, Valéria Tessaro; Santos Paulino, Sarah Gondim; Villa, Bárbara De; Zembruski, Bianca Modena; Forcelini, Cassiano Matheus; Hahn, Siomara Regina; Antunes, Marina Venzon; Linden, Rafael.
Affiliation
  • Müller IR; Analytical Toxicology Laboratory, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.
  • Grandi VT; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.
  • Santos Paulino SG; College of Medicine, Universidade de Passo Fundo, Rua Teixeira Soares, 817, Passo Fundo, RS, CEP, 99010-080, Brazil.
  • Villa B; College of Medicine, Universidade de Passo Fundo, Rua Teixeira Soares, 817, Passo Fundo, RS, CEP, 99010-080, Brazil.
  • Zembruski BM; College of Pharmacy, Universidade de Passo Fundo, Rodovia BR 285, Km 293, Passo Fundo, RS, CEP, 99052-900, Brazil.
  • Forcelini CM; College of Pharmacy, Universidade de Passo Fundo, Rodovia BR 285, Km 293, Passo Fundo, RS, CEP, 99052-900, Brazil.
  • Hahn SR; Hospital São Vicente de Paulo, Rua Uruguai, 2050, Passo Fundo-RS, CEP, 99010-111, Brazil.
  • Antunes MV; College of Pharmacy, Universidade de Passo Fundo, Rodovia BR 285, Km 293, Passo Fundo, RS, CEP, 99052-900, Brazil.
  • Linden R; Analytical Toxicology Laboratory, Universidade Feevale, Rua Rubem Berta, 200, Novo Hamburgo, RS, CEP, 93525-080, Brazil.
Bioanalysis ; 16(9): 415-427, 2024.
Article in En | MEDLINE | ID: mdl-38497729
ABSTRACT

Background:

Fingerprint drug concentrations can be used as a noninvasive and convenient alternative to evaluate adherence to pharmacotherapy.

Methods:

Fingerprints were applied over glass slides, extracted and analyzed by ultra-high performance LC-MS/MS. The assay and drug adherence questionnaires were applied to 30 epilepsy patients.

Results:

The assay had linearity in the range 0.05-10 ng fingerprint-1, with precision of 2.16-7.9% and accuracy of 95.0-102.8%. Carbamazepine (CBZ) levels in fingerprints were stable at 45°C for 15 days. Concentrations in patient samples were 0.06-9.28 ng fingerprint-1. A significant difference (p = 0.003) was found between CBZ concentrations in fingerprints between patient groups divided as low and medium/high adherence.

Conclusion:

This method can potentially be applied to the identification of epilepsy patients with low adherence to CBZ pharmacotherapy.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carbamazepine / Feasibility Studies / Epilepsy / Tandem Mass Spectrometry / Medication Adherence Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: Bioanalysis Year: 2024 Document type: Article
Fulltext
Print
XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carbamazepine / Feasibility Studies / Epilepsy / Tandem Mass Spectrometry / Medication Adherence Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: Bioanalysis Year: 2024 Document type: Article