Pulsed electromagnetic field-assisted reduced graphene oxide composite 3D printed nerve scaffold promotes sciatic nerve regeneration in rats.
Biofabrication
; 16(3)2024 May 07.
Article
in En
| MEDLINE
| ID: mdl-38604162
ABSTRACT
Peripheral nerve injuries can lead to sensory or motor deficits that have a serious impact on a patient's mental health and quality of life. Nevertheless, it remains a major clinical challenge to develop functional nerve conduits as an alternative to autologous grafts. We applied reduced graphene oxide (rGO) as a bioactive conductive material to impart electrophysiological properties to a 3D printed scaffold and the application of a pulsed magnetic field to excite the formation of microcurrents and induce nerve regeneration.In vitrostudies showed that the nerve scaffold and the pulsed magnetic field made no effect on cell survival, increased S-100ßprotein expression, enhanced cell adhesion, and increased the expression level of nerve regeneration-related mRNAs.In vivoexperiments suggested that the protocol was effective in promoting nerve regeneration, resulting in functional recovery of sciatic nerves in rats, when they were damaged close to that of the autologous nerve graft, and increased expression of S-100ß, NF200, and GAP43. These results indicate that rGO composite nerve scaffolds combined with pulsed magnetic field stimulation have great potential for peripheral nerve rehabilitation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sciatic Nerve
/
Rats, Sprague-Dawley
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Electromagnetic Fields
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Tissue Scaffolds
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Printing, Three-Dimensional
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Graphite
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Nerve Regeneration
Limits:
Animals
Language:
En
Journal:
Biofabrication
Year:
2024
Document type:
Article