Target analysis of psychoactive drugs in oral fluid by QuEChERS extraction and LC-MS/MS.
J Pharm Biomed Anal
; 244: 116139, 2024 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-38608509
ABSTRACT
This study aimed to validate a modified QuEChERS method, followed by liquid chromatography-tandem mass spectrometry, for the determination of 51 psychoactive substances and screening of 22 ones in oral fluid from electronic dance music party (EDM) attendees. Unstimulated oral fluid was collected in a polypropylene tube and stored in a glass vial at -20 ºC. The sample was extracted with acetonitrilewater and MgSO4/NaOAc, followed by cleanup with primary secondary amine and MgSO4. The effectiveness of the sample storage conditions was shown to be comparable to when the Quantisal™ buffer was used, with no substantial concentration loss (< 15%) for all the substances after up to 72â¯hours at -20º C. The method was satisfactorily validated, with limits of detection (LOD) and quantification (LOQ) ranging from 0.04 to 0.5â¯ng/mL and 0.1-1.5â¯ng/mL, respectively, and was applied to the analysis of 62 real samples. The main substances detected were 3,4-methylenedioxymethamphetamine (MDMA) (<0.5-829â¯ng/mL) and/or methylenedioxyamphetamine (MDA) (10.1 - 460.6â¯ng/mL), found in 27 samples, and cocaine (13.0-407.3â¯ng/mL) and its metabolites (benzoylecgonine 0.17-214.1â¯ng/mL; ecgonine methyl ester 1.8-150.1â¯ng/mL) in eight samples. Methamphetamine (11-439â¯ng/mL) was detected in eight samples, along with MDMA and MDA; eutylone was detected in two cases (4.7 and 24.1â¯ng/mL) reported as "ecstasy" ingestion. A comparison between self-reported drug use and results of oral fluid analysis indicated that the use of illicit substances is often underreported among EDM attendees, who are often unaware of the substances they consume.
Key words
Full text:
1
Collection:
01-internacional
Health context:
1_ASSA2030
Database:
MEDLINE
Main subject:
Psychotropic Drugs
/
Saliva
/
Substance Abuse Detection
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Limit of Detection
Limits:
Humans
Language:
En
Journal:
J Pharm Biomed Anal
/
J. pharm. biomed. anal
/
Journal of pharmaceutical and biomedical analysis
Year:
2024
Document type:
Article