Ruixingdingia sedimenti gen. nov., sp. nov., a novel taxon within the family Paracoccaceae isolated from sediment of Qiantang River.
Int J Syst Evol Microbiol
; 74(4)2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38668744
ABSTRACT
A Gram-stain-negative bacterium, designated LG-4T, was isolated from sediment of Qiantang River in Zhejiang Province, PR China. Cells were strictly aerobic, non-spore-forming, non-motile and short-rod-shaped (1.0-1.2 µm long and 0.7-0.8 µm wide). Growth occurred at 15-42â°C (optimum, 30â°C), at pH 5.0-9.0 (pH 7.0) and at 0-2.0â% (w/v) NaCl (optimum, 0.5â% NaCl). Strain LG-4T showed 95.75-96.90â% 16S rRNA gene sequence similarity to various type strains of the genera Tabrizicola, Pseudotabrizicola, Phaeovulum, Rhodobacter and Wagnerdoeblera of the family Paracoccaceae, and the most closely related strain was Tabrizicola soli ZQBWT (96.90â% similarity). The phylogenomic tree showed that strain LG-4T clustered in the family Paracoccaceae and was positioned outside of the clade composed of the genera Wagnerdoeblera and Falsigemmobacter. The average nucleotide identity and digital DNA-DNA hybridization values between strain LG-4T and the related type strains were in the range of 74.19-77.56â% and 16.70-25.80â%, respectively. The average amino acid identity (AAI) values between strain LG-4T and related type strains of the family Paracoccaceae were 60.94-69.73â%, which are below the genus boundary (70â%). The evolutionary distance (ED) values between LG-4T and the related genera of the family Paracoccaceae were 0.21-0.34, which are within the recommended standard (≥0.21-0.23) for defining a novel genus in the family Paracoccaceae. The predominant cellular fatty acids were C18ââ1 ω7c, C19ââ0 cyclo ω8c, C18ââ0 and C16ââ0, the isoprenoid quinone was Q-10, and the major polar lipids were phospholipid, phosphatidylglycerol, phosphatidylcholine, aminolipid and two unknown polar lipids. The genome size was 4.7 Mb with 68.6 mol% G+C content. On the basis of distinct phylogenetic relationships, low AAI values and high ED values, and differential phenotypic, physiological and biochemical characteristics, strain LG-4T represents a novel species of a new genus in the family Paracoccaceae, for which the name Ruixingdingia sedimenti gen. nov., sp. nov. is proposed. The type strain is LG-4T (=MCCC 1K08849T=KCTC 8136T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Base Composition
/
DNA, Bacterial
/
RNA, Ribosomal, 16S
/
Bacterial Typing Techniques
/
Sequence Analysis, DNA
/
Geologic Sediments
/
Rivers
/
Fatty Acids
/
Nucleic Acid Hybridization
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Year:
2024
Document type:
Article