Your browser doesn't support javascript.
loading
Identification of candidate genes for adult plant stripe rust resistance transferred from Aegilops ventricosa 2NvS into wheat via fine mapping and transcriptome analysis.
Wang, Yuqi; Gao, Mengru; Jiang, Yunfeng; Huang, Wuzhou; Zhao, Xin; Zhu, Wei; Li, Hao; Wang, Yi; Zeng, Jian; Wu, Dandan; Wei, Yuming; Zhou, Yonghong; Zheng, Youliang; Zhang, Peng; Chen, Guoyue; Kang, Houyang.
Affiliation
  • Wang Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Gao M; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Jiang Y; Plant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Cobbitty, NSW, 2570, Australia.
  • Huang W; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Zhao X; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Zhu W; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Li H; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Wang Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Zeng J; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Wu D; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Wei Y; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Zhou Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Zheng Y; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Zhang P; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Chen G; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
  • Kang H; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Theor Appl Genet ; 137(5): 116, 2024 May 02.
Article in En | MEDLINE | ID: mdl-38698276
ABSTRACT
KEY MESSAGE An adult plant gene for resistance to stripe rust was narrowed down to the proximal one-third of the 2NvS segment translocated from Aegilops ventricosa to wheat chromosome arm 2AS, and based on the gene expression analysis, two candidate genes were identified showing a stronger response at the adult plant stage compared to the seedling stage. The 2NvS translocation from Aegilops ventricosa, known for its resistance to various diseases, has been pivotal in global wheat breeding for more than three decades. Here, we identified an adult plant resistance (APR) gene in the 2NvS segment in wheat line K13-868. Through fine mapping in a segregating near-isogenic line (NIL) derived population of 6389 plants, the candidate region for the APR gene was narrowed down to between 19.36 Mb and 33 Mb in the Jagger reference genome. Transcriptome analysis in NILs strongly suggested that this APR gene conferred resistance to stripe rust by triggering plant innate immune responses. Based on the gene expression analysis, two disease resistance-associated genes within the candidate region, TraesJAG2A03G00588940 and TraesJAG2A03G00590140, exhibited a stronger response to Puccinia striiformis f. sp. tritici (Pst) infection at the adult plant stage than at the seedling stage, indicating that they could be potential candidates for the resistance gene. Additionally, we developed a co-dominant InDel marker, InDel_31.05, for detecting this APR gene. Applying this marker showed that over one-half of the wheat varieties approved in 2021 and 2022 in Sichuan province, China, carry this gene. Agronomic trait evaluation of NILs indicated that the 2NvS segment effectively mitigated the negative effects of stripe rust on yield without affecting other important agronomic traits. This study provided valuable insights for cloning and breeding through the utilization of the APR gene present in the 2NvS segment.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Basidiomycota / Triticum / Chromosome Mapping / Genes, Plant / Gene Expression Profiling / Disease Resistance / Aegilops Language: En Journal: Theor Appl Genet Year: 2024 Document type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Basidiomycota / Triticum / Chromosome Mapping / Genes, Plant / Gene Expression Profiling / Disease Resistance / Aegilops Language: En Journal: Theor Appl Genet Year: 2024 Document type: Article