In silico identification and expression analysis of superoxide dismutases in Tenebrio molitor.
Genes Genomics
; 46(7): 733-742, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38700830
ABSTRACT
BACKGROUND:
Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage.OBJECTIVE:
To investigate expressions of SODs under oxidative stress in Tenebrio molitor.METHODS:
Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR.RESULTS:
Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu2+ binding site, Zn2+ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs.CONCLUSION:
Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Superoxide Dismutase
/
Tenebrio
/
Oxidative Stress
Limits:
Animals
Language:
En
Journal:
Genes Genomics
Year:
2024
Document type:
Article