Glycoproteomics: Charting new territory in mass spectrometry and glycobiology.
J Mass Spectrom
; 59(6): e5034, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38726698
ABSTRACT
Glycosylation is an incredibly common and diverse post-translational modification that contributes widely to cellular health and disease. Mass spectrometry is the premier technique to study glycoproteins; however, glycoproteomics has lagged behind traditional proteomics due to the challenges associated with studying glycosylation. For instance, glycans dissociate by collision-based fragmentation, thus necessitating electron-based fragmentation for site-localization. The vast glycan heterogeneity leads to lower overall abundance of each glycopeptide, and often, ion suppression is observed. One of the biggest issues facing glycoproteomics is the lack of reliable software for analysis, which necessitates manual validation and serves as a massive bottleneck in data processing. Here, I will discuss each of these challenges and some ways in which the field is attempting to address them, along with perspectives on how I believe we should move forward.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Glycoproteins
/
Proteomics
/
Glycomics
Limits:
Animals
/
Humans
Language:
En
Journal:
J Mass Spectrom
Year:
2024
Document type:
Article