Poppy Seed Allergy: Molecular Diagnosis and Cross-Reactivity With Tree Nuts.
J Allergy Clin Immunol Pract
; 12(8): 2144-2154.e11, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-38734371
ABSTRACT
BACKGROUND:
Poppy seed (PS) can be a cause of severe allergic reactions, especially in individuals with concurrent allergy to tree nuts and other seeds, but diagnostic criteria and sensitization patterns are lacking.OBJECTIVE:
To assess the role of PS extract and individual allergens in diagnosing PS allergy and their cross-reactivities with tree nuts and buckwheat.METHODS:
Our retrospective study included 36 PS-sensitized patients; 10 with a positive and 26 with a negative oral food challenge (OFC). We identified individual PS allergens and compared the diagnostic performance of specific IgE (sIgE) to PS extract with its allergens. Cross-reactivities between PS and related allergens from other seeds were assessed by a competitive enzyme-linked immunosorbent assay.RESULTS:
We identified 4 novel PS allergens Pap s 1 (vicilin), Pap s 1 (27-424) (α-hairpinin), Pap s 2 (legumin), and Pap s 3 (small hydrophilic seed protein). A positive OFC correlated with higher PS-sIgE levels and elevated sIgE levels for the PS allergens, except for Pap s 3. PS and α-hairpinin-sIgE effectively differentiated allergic from tolerant patients, with area under the curve values of 0.95 and 0.94. PS-sIgE >10.00 kUA/L exhibited 90% sensitivity and 73% specificity, whereas α-hairpinin-sIgE >2.60 kUA/L showed 100% sensitivity and 77% specificity. PS vicilin and legumin highly cross-reacted with hazelnut and buckwheat homologs, whereas α-hairpinin-sIgE cross-reacted with the related almond allergen.CONCLUSIONS:
This is the most extensive study on PS allergy to date. PS and α-hairpinin-sIgE are highly sensitive indicators of clinical reactivity to PS, whereas vicilin and legumin-sIgE contribute to concurrent sensitization to hazelnut and buckwheat.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seeds
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Immunoglobulin E
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Allergens
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Cross Reactions
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Nut Hypersensitivity
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Seed Storage Proteins
Limits:
Adolescent
/
Adult
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Child
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Female
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Humans
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Male
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Middle aged
Language:
En
Journal:
J Allergy Clin Immunol Pract
Year:
2024
Document type:
Article