Microbial Synthetic Epigenetic Tools Design and Applications.
ACS Synth Biol
; 13(6): 1621-1632, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38758631
ABSTRACT
Microbial synthetic epigenetics offers significant opportunities for the design of synthetic biology tools by leveraging reversible gene control mechanisms without altering DNA sequences. However, limited understanding and a lack of technologies for thorough analysis of the mechanisms behind epigenetic modifications have hampered their utilization in biotechnological applications. In this review, we explore advancements in developing epigenetic-based synthetic gene regulatory tools at both transcriptional and post-transcriptional levels. Furthermore, we examine strategies developed to construct epigenetic-based circuits that provide controllable and stable gene regulation, aiming to boost the performance of microbial chassis cells. Finally, we discuss the current challenges and perspectives in the development of synthetic epigenetic tools.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Epigenesis, Genetic
/
Synthetic Biology
Language:
En
Journal:
ACS Synth Biol
Year:
2024
Document type:
Article