SIRT6 positively regulates antiviral response in a bony fish, the Chinese perch Siniperca chuatsi.
Fish Shellfish Immunol
; 150: 109662, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38821229
ABSTRACT
SIRT6, a key member of the sirtuin family, plays a pivotal role in regulating a number of vital biological processes, including energy metabolism, oxidative stress, and immune system modulation. Nevertheless, the function of SIRT6 in bony fish, particularly in the context of antiviral immune response, remains largely unexplored. In this study, a sirt6 was cloned and characterized in a commercial fish, the Chinese perch (Siniperca chuatsi). The SIRT6 possesses conserved SIR2 domain with catalytic core region when compared with other vertebrates. Tissue distribution analysis indicated that sirt6 was expressed in all detected tissues, and the sirt6 was significantly induced following infection of infectious haemorrhagic syndrome virus (IHSV). The overexpression of SIRT6 resulted in significant upregulation of interferon-stimulated genes (ISGs), such as viperin, mx, isg15, irf3 and ifp35, and inhibited viral replication. It was further found that SIRT6 was located in nucleus and could enhance the expression of ISGs induced by type I and II IFNs. These findings may provide new information in relation with the function of SIRT6 in vertebrates, and with viral prevention strategy development in aquaculture.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Perches
/
Gene Expression Regulation
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Amino Acid Sequence
/
Rhabdoviridae Infections
/
Fish Proteins
/
Sirtuins
/
Fish Diseases
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Immunity, Innate
Limits:
Animals
Language:
En
Journal:
Fish Shellfish Immunol
Year:
2024
Document type:
Article