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Reduction of ristocetin-induced platelet aggregation (RIPA) during storage despite plasma renewal: evidence for hemostatic importance of GPIbα shedding.
Hosseini, Ehteramolsadat; Taherabadi, Emad; Rajabi, Ali; Ghasemzadeh, Mehran.
Affiliation
  • Hosseini E; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Taherabadi E; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Rajabi A; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Ghasemzadeh M; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Expert Rev Hematol ; 17(7): 391-403, 2024 Jul.
Article in En | MEDLINE | ID: mdl-38889268
ABSTRACT

BACKGROUND:

Platelet storage is complicated by deleterious changes, among which reduction of ristocetin-induced platelet aggregation (RIPA) has a poorly understood mechanism. The study elucidates the mechanistic roles of all the possible players in this process. RESEARCH DESIGN AND

METHODS:

PRP-platelet concentrates were subjected to RIPA, collagen-induced platelet aggregation (CIPA), and flowcytometric analysis of GPIbα and PAC-1 binding from days 0 to 5 of storage. Platelet-poor plasma was subjected to colorimetric assays for glucose/LDH evaluation and automatic analyzer to examine VWF antigen and activity.

RESULTS:

From day three of platelet storage, reducing CIPA but not RIPA was correlated with the reduction of both metabolic state and integrin activity. RIPA reduction was directly related to the decreased levels of total-content/expression of GPIbα, and inversely related to its shedding levels during storage. Re-suspension of 5-day stored platelet in fresh plasma compensated CIPA, but not RIPA. VWF concentration and its activity did not change during storage while they had no correlation with RIPA.

CONCLUSIONS:

This study identified the irreversible loss of platelet GPIbα, but not VWF status, as the primary cause of the storage-dependent decrease of RIPA. Unlike CIPA, this observation was not compensated by plasma refreshment, suggesting that some evidence of PSL may not be recovered after transfusion.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Platelets / Blood Preservation / Von Willebrand Factor / Ristocetin / Platelet Aggregation / Platelet Glycoprotein GPIb-IX Complex Limits: Humans Language: En Journal: Expert Rev Hematol Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Platelets / Blood Preservation / Von Willebrand Factor / Ristocetin / Platelet Aggregation / Platelet Glycoprotein GPIb-IX Complex Limits: Humans Language: En Journal: Expert Rev Hematol Year: 2024 Document type: Article