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Molecular diversity and polyparasitism of avian trypanosomes in the Brazilian Atlantic Rainforest.
Duarte, Rodrigo G; Jardim, Talys Henrique A; Paulino, Patrícia G; Dias, Roberto J P; Rossi, Mariana F; D Agosto, Marta; Peixoto, Maristela P; Guedes Junior, Daniel S; Gonçalves, Natalia P; Massard, Carlos L; Santos, Huarrisson A.
Affiliation
  • Duarte RG; Universidade Federal Rural do Rio de Janeiro/UFRRJ, Instituto de Veterinária, Departamento de Epidemiologia e Saúde Pública, BR 465, Km 07, 23897-000 Seropédica, RJ, Brazil.
  • Jardim THA; Universidade Federal Rural do Rio de Janeiro/UFRRJ, Instituto de Veterinária, Departamento de Epidemiologia e Saúde Pública, BR 465, Km 07, 23897-000 Seropédica, RJ, Brazil.
  • Paulino PG; Universidade Federal Rural do Rio de Janeiro/UFRRJ, Instituto de Veterinária, Departamento de Epidemiologia e Saúde Pública, BR 465, Km 07, 23897-000 Seropédica, RJ, Brazil.
  • Dias RJP; Universidade Federal de Juiz de Fora/UFJF, Instituto de Ciências Biológicas, Departamento de Zoologia, Rua José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
  • Rossi MF; Universidade Federal de Juiz de Fora/UFJF, Instituto de Ciências Biológicas, Departamento de Zoologia, Rua José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
  • D Agosto M; Universidade Federal de Juiz de Fora/UFJF, Instituto de Ciências Biológicas, Departamento de Zoologia, Rua José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
  • Peixoto MP; Universidade Federal Rural do Rio de Janeiro/UFRRJ, Instituto de Veterinária, Departamento de Parasitologia Animal, BR 465, Km 07, 23897-000 Seropédica, RJ, Brazil.
  • Guedes Junior DS; Fundação Oswaldo Cruz/FIOCRUZ, Instituto de Tecnologia em Imunobiológicos, Departamento de Controle de Qualidade, Av. Brasil, 4365, Manguinhos, 21040-090 Rio de Janeiro, RJ, Brazil.
  • Gonçalves NP; Fundação Oswaldo Cruz/FIOCRUZ, Instituto de Tecnologia em Imunobiológicos, Departamento de Controle de Qualidade, Av. Brasil, 4365, Manguinhos, 21040-090 Rio de Janeiro, RJ, Brazil.
  • Massard CL; Universidade Federal Rural do Rio de Janeiro/UFRRJ, Instituto de Veterinária, Departamento de Parasitologia Animal, BR 465, Km 07, 23897-000 Seropédica, RJ, Brazil.
  • Santos HA; Universidade Federal Rural do Rio de Janeiro/UFRRJ, Instituto de Veterinária, Departamento de Epidemiologia e Saúde Pública, BR 465, Km 07, 23897-000 Seropédica, RJ, Brazil.
An Acad Bras Cienc ; 96(3): e20230629, 2024.
Article in En | MEDLINE | ID: mdl-38922254
ABSTRACT
The current study proposes to investigate the diversity and phylogeny of trypanosomes parasitizing wild birds from the Brazilian Atlantic Forest. Cytological examination was carried out by light microscopy of blood smears and positive birds were selected for amplification of the 18S rDNA sequence through PCR. The resulting amplicons were subjected to purification, cloning, and sequencing analysis. Phylogenetic reconstruction was conducted, including all avian trypanosomes representative's lineages. A total of ten bird samples from species of Turdus flavipes (N=1/12), T. albicollis (N=1/8), Tachyphonus coronatus (N=6/121), Thamnophilus caerulescens (N=1/22) and Synallaxis spixi (N=1/8) were positive for Trypanosoma spp. In the six specimens of T. coronatus, five distinct lineages of Trypanosoma spp. 18S-rRNA were observed in ninety sequences obtained, and using the strategy of cloning independent PCR, it was possible to observe that two of them were related to T. avium (JB01/JB02), and three were closed related to T. bennetti (JB03/ JB04/JB05). Addionaly, all fifteen sequences obtained from T. caerulescens/ S. spixi/T. flavipes/T. albicollis were identical. The present research is the first study to access molecular diversity and polyparasitism by avian trypanosomes in Brazil. The current research exhibits the wide genetic variability in avian trypanosomes and its non-specific relationship with its avian hosts.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phylogeny / Trypanosoma / Birds / Polymerase Chain Reaction Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: An Acad Bras Cienc Year: 2024 Document type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phylogeny / Trypanosoma / Birds / Polymerase Chain Reaction Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: An Acad Bras Cienc Year: 2024 Document type: Article