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Extraction of Proteins from Marine Microbial Communities Sampled by Lab Filters.
Williams, Timothy J; Cavicchioli, Ricardo.
Affiliation
  • Williams TJ; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, Australia. tim.williams@uts.edu.au.
  • Cavicchioli R; School of Life Sciences, University of Technology Sydney, Sydney, Australia. tim.williams@uts.edu.au.
Methods Mol Biol ; 2820: 1-6, 2024.
Article in En | MEDLINE | ID: mdl-38941009
ABSTRACT
A method for the recovery of whole-cell protein extracts from biomass on membrane filters is provided here. The protein extraction method is ideal for biomass captured by filtration of large water volumes, including seawater from marine environments. The protein extraction method includes both chemical disruption and physical disruption to lyse cells and release protein for subsequent metaproteomic analysis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Seawater / Filtration Language: En Journal: Methods Mol Biol Year: 2024 Document type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Seawater / Filtration Language: En Journal: Methods Mol Biol Year: 2024 Document type: Article