[High expression of the stemness-associated molecule Nanog in esophageal squamous cell carcinoma tissues promotes tumor invasion and metastasis by activating the TGF-ß signaling pathway].
Nan Fang Yi Ke Da Xue Xue Bao
; 44(6): 1209-1216, 2024 Jun 20.
Article
in Zh
| MEDLINE
| ID: mdl-38977352
ABSTRACT
OBJECTIVE:
To investigate the expression of Nanog and its regulatory relationship with MMP-2/MMP-9 proteins in esophageal squamous cell carcinoma (ESCC).METHODS:
We detected Nanog and MMP-2/MMP-9 protein expressions in 127 ESCC tissues and 82 adjacent normal tissues using immunohistochemistry and explored their correlations with the clinicopathological parameters and prognosis of the patients. GEO database was utilized to analyze the pathways enriched with the stemness-related molecules including Nanog, and TIMER online tool was used to analyze the correlations among TßR1, MMP-2, and MMP-9 in esophageal cancer.RESULTS:
Nanog and MMP-2/MMP-9 proteins were significantly upregulated in ESCC tissues and positively intercorrelated. Their expression levels were closely correlated with infiltration depth and lymph node metastasis of ESCC but not with age, gender, or tumor differentiation. The patients with high expressions of Nanog and MMP-2/MMP-9 had significantly shorter survival time. Bioinformatics analysis showed enrichment of stemness-associated molecules in the TGF-ß signaling pathway, and the expressions of MMP-2/MMP-9 and TßR1 were positively correlated. In cultured ESCC cells, Nanog knockdown significantly decreased the expression of TßR1, p-Smad2/3, MMP-2, and MMP-9 and strongly inhibited cell migration.CONCLUSION:
The high expressions of Nanog, MMP-2, and MMP-9, which are positively correlated, are closely related with invasion depth, lymph node metastasis, and prognosis of ESCC. Nanog regulates the expressions of MMP-2/MMP-9 proteins through the TGF-ß signaling pathway, and its high expression promotes migration of ESCC cells.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Esophageal Neoplasms
/
Signal Transduction
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Transforming Growth Factor beta
/
Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9
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Nanog Homeobox Protein
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Esophageal Squamous Cell Carcinoma
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Lymphatic Metastasis
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Neoplasm Invasiveness
Limits:
Female
/
Humans
/
Male
Language:
Zh
Journal:
Nan Fang Yi Ke Da Xue Xue Bao
/
Nanfang Yike Daxue xuebao
Year:
2024
Document type:
Article