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Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024.
Beck, Andrew S; Lopareva, Elena N; Hwang, Hyun; Hart, Derek; de Almeida, Marcos; Anderson, Raydel; Rota, Paul A; Bankamp, Bettina.
Affiliation
  • Beck AS; Viral Vaccine Preventable Diseases Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, United States.
  • Lopareva EN; Viral Vaccine Preventable Diseases Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, United States.
  • Hwang H; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, United States.
  • Hart D; ASRT Inc, Atlanta, United States.
  • de Almeida M; Strategic Innovative Solutions Inc., Clearwater, United States.
  • Anderson R; Viral Vaccine Preventable Diseases Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, United States.
  • Rota PA; Viral Vaccine Preventable Diseases Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, United States.
  • Bankamp B; Viral Vaccine Preventable Diseases Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, United States.
Euro Surveill ; 29(28)2024 Jul.
Article in En | MEDLINE | ID: mdl-38994600
ABSTRACT
We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensitivity of the assay.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Viral / Sensitivity and Specificity / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Measles / Measles virus Limits: Humans Language: En Journal: Euro Surveill Year: 2024 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Viral / Sensitivity and Specificity / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Measles / Measles virus Limits: Humans Language: En Journal: Euro Surveill Year: 2024 Document type: Article