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Isolation, expansion, and adoptive transfer of human ILC2s for the treatment of mice bearing liquid and solid tumors.
Li, Zhenlong; Ma, Rui; Tang, Hejun; Caligiuri, Michael A; Yu, Jianhua.
Affiliation
  • Li Z; Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA.
  • Ma R; Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA.
  • Tang H; Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA.
  • Caligiuri MA; Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA; City of Hope Comprehensive Cancer Center, Los Angeles,
  • Yu J; Department of Hematology & Hematopoietic Cell Transplantation, City of Hope National, Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA; City of Hope Comprehensive Cancer Center, Los Angeles,
STAR Protoc ; 5(3): 103096, 2024 Sep 20.
Article in En | MEDLINE | ID: mdl-39088329
ABSTRACT
Type 2 innate lymphoid cells (ILC2s) are crucial in regulating immune responses and various physiological processes, including tissue repair, metabolic homeostasis, inflammation, and cancer surveillance. Here, we present a protocol that outlines the isolation, expansion, and adoptive transfer of human ILC2s from peripheral blood mononuclear cells for an in vivo lineage tracking experiment in a mouse model. Additionally, we detail the steps involved in the adoptive transfer of human ILC2s to recipient mice bearing human liquid or solid tumors. For complete details on the use and execution of this protocol, please refer to Li et al.1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lymphocytes / Adoptive Transfer / Immunity, Innate / Neoplasms Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lymphocytes / Adoptive Transfer / Immunity, Innate / Neoplasms Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article