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[Role and mechanism of ginsenoside Rg1 in ameliorating sepsis-induced acute lung injury based on PERK/eIF2α/ATF4/CHOP-induced alveolar epithelial cell apoptosis].
Zhong, Kai-Qiang; Huang, Yin-Gui; Chen, Xiu-Ping; Chen, Rui; Wu, Chu-Wen; Zou, Jia-Zhen; Xi, Xiao-Tu; Li, Jun; Yan, Chun-Jiang.
Affiliation
  • Zhong KQ; Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510120, China.
  • Huang YG; Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510120, China.
  • Chen XP; Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China.
  • Chen R; Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China Guangdong Provincial Key Laboratory of Research on Emergency in Traditional Chinese Medicine Guangzhou 510006, China.
  • Wu CW; School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University Guangzhou 510006, China.
  • Zou JZ; School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University Guangzhou 510006, China.
  • Xi XT; the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510120, China Guangdong Provincial Key Laboratory of Research on Emergency in Traditional Chinese Medicine Guangzhou 510006, China.
  • Li J; the First Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510405, China.
  • Yan CJ; the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510120, China.
Zhongguo Zhong Yao Za Zhi ; 49(14): 3837-3847, 2024 Jul.
Article in Zh | MEDLINE | ID: mdl-39099357
ABSTRACT
The study investigates the therapeutic effects and mechanisms of ginsenoside Rg_1(GRg_1) on sepsis-induced acute lung injury(SALI). A murine model of SALI was created using cecal ligation and puncture(CLP) surgery, and mice were randomly assigned to groups for GRg_1 intervention. Survival and body weight changes were recorded, lung function was assessed with a non-invasive lung function test system, and lung tissue damage was evaluated through HE staining. The content and expression of inflammatory factors were measured by ELISA and qRT-PCR. Apoptosis was examined using flow cytometry and TUNEL staining. The activation and expression of apoptosis-related molecules cysteinyl aspartate specific proteinase 3(caspase-3), B-cell lymphoma-2(Bcl-2), Bcl-2 associated X protein(Bax), and endoplasmic reticulum stress-related molecules protein kinase R-like endoplasmic reticulum kinase(PERK), eukaryotic initiation factor 2α(eIF2α), activating transcription factor 4(ATF4), and C/EBP homologous protein(CHOP) were studied using Western blot and qRT-PCR. In addition, an in vitro model of lipopolysaccharide(LPS)-induced lung alveolar epithelial cell injury was used, with the application of the endoplasmic reticulum stress inducer tunicamycin to validate the action mechanism of GRg_1.

RESULTS:

indicated that, when compared to the model group, GRg_1 intervention significantly enhanced the survival time of CLP mice, mitigated body weight loss, and improved impaired lung function indices. The GRg_1-treated mice also displayed reduced lung tissue pathological scores, a reduced lung tissue wet-to-dry weight ratio, and lower protein content in the bronchoalveolar lavage fluid. Serum levels of interleukin-6(IL-6), interleukin-1ß(IL-1ß), and tumor necrosis factor-α(TNF-α), as well as the mRNA expressions of these cytokines in lung tissues, were decreased. There was a notable decrease in the proportion of apopto-tic alveolar epithelial cells, and down-regulated expressions of caspase-3, Bax, PERK, eIF2α, ATF4, and CHOP and up-regulated expression of Bcl-2 were observed. In vitro findings showed that the apoptosis-lowering and apoptosis-related protein down-regulating effects of GRg_1 were significantly inhibited with the co-application of tunicamycin. Altogether, GRg_1 reduces apoptosis of alveolar epithelial cells, inhibits inflammation in the lungs, alleviates lung injury, and enhances lung function, possibly through the PERK/eIF2α/ATF4/CHOP pathway.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Eukaryotic Initiation Factor-2 / Apoptosis / Sepsis / EIF-2 Kinase / Ginsenosides / Activating Transcription Factor 4 / Transcription Factor CHOP / Acute Lung Injury / Alveolar Epithelial Cells Limits: Animals / Humans / Male Language: Zh Journal: Zhongguo Zhong Yao Za Zhi Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Eukaryotic Initiation Factor-2 / Apoptosis / Sepsis / EIF-2 Kinase / Ginsenosides / Activating Transcription Factor 4 / Transcription Factor CHOP / Acute Lung Injury / Alveolar Epithelial Cells Limits: Animals / Humans / Male Language: Zh Journal: Zhongguo Zhong Yao Za Zhi Year: 2024 Document type: Article