Inferring clonal somatic mutations directed by X chromosome inactivation status in single cells.
Genome Biol
; 25(1): 214, 2024 Aug 09.
Article
in En
| MEDLINE
| ID: mdl-39123248
ABSTRACT
Analysis of clonal dynamics in human tissues is enabled by somatic genetic variation. Here, we show that analysis of mitochondrial mutations in single cells is dramatically improved in females when using X chromosome inactivation to select informative clonal mutations. Applying this strategy to human peripheral mononuclear blood cells reveals clonal structures within T cells that otherwise are blurred by non-informative mutations, including the separation of gamma-delta T cells, suggesting this approach can be used to decipher clonal dynamics of cells in human tissues.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
X Chromosome Inactivation
/
Single-Cell Analysis
/
Mutation
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Genome Biol
Year:
2024
Document type:
Article