Removal of signal peptide variants by cation exchange chromatography: A case study.
Protein Expr Purif
; 225: 106581, 2025 Jan.
Article
in En
| MEDLINE
| ID: mdl-39168393
ABSTRACT
Signal peptide (SP) is required for secretion of recombinant proteins and typically cleaved by signal peptidase at its C-region to generate the mature proteins. Miscleavage of the SP is reported occasionally, resulting in a truncated- or elongated-terminal sequence. In the present work, we demonstrated that cation exchange (CEX) chromatography is an effective means for removing SP variants with a case study. With the selected resin/conditions, the chromatographic performance is comparable between runs performed at the low end and high end of load density and elution range. The procedure described in this work can be used as a general approach for resin selection and optimization of chromatographic conditions to remove byproducts that bind more strongly than the product to the selected resin.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Sorting Signals
Language:
En
Journal:
Protein Expr Purif
/
Protein expr. purif
/
Protein expression and purification
Year:
2025
Document type:
Article