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Smartphone-assisted colorimetric biosensor for the rapid visual detection of natural antioxidants in food samples.
Tang, Jun; Gao, Zhenyu; Xu, Longfei; Zhao, Qianqian; Hu, Tianfeng; Luo, Yongfeng; Dou, Jinkang; Bai, Yuanjuan; Xia, Liaoyuan; Du, Kun.
Affiliation
  • Tang J; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Gao Z; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Xu L; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Zhao Q; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Hu T; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Luo Y; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Dou J; Department of Energetic Materials Science and Technology, Xi'an Modern Chemistry Research Institute, Xi'an 710065, China.
  • Bai Y; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Xia L; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.
  • Du K; Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China. Electronic address: dukun@csuft.edu.cn.
Food Chem ; 462: 141026, 2025 Jan 01.
Article in En | MEDLINE | ID: mdl-39216373
ABSTRACT
Quantitative monitoring of the concentrations of epigallocatechin gallate (EGCG) and cysteine (Cys) is of great significance for promoting human health. In this study, iron/aluminum bimetallic MOF material MIL-53 (Fe, Al) was rapidly prepared under room temperature using a co-precipitation method, followed by investigating the peroxidase-like (POD-like) activity of MIL-53(Fe, Al) using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic substrate. The results showed that the Michaelis -Menten constants of TMB and H2O2 as substrates were 0.167 mM and 0.108 mM, respectively. A colorimetric sensing platform for detecting EGCG and Cys was developed and successfully applied for analysis and quantitative detection using a smartphone. The linear detection range for EGCG was 15∼80 µM (R2=0.994) and for Cys was 7∼95 µM (R2=0.998). The limits of detection (LOD) were 0.719 µM and 0.363 µM for EGCG and Cys, respectively. This work provides a new and cost-effective approach for the real-time analysis of catechins and amino acids.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques / Catechin / Colorimetry / Smartphone / Antioxidants Language: En Journal: Food Chem Year: 2025 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques / Catechin / Colorimetry / Smartphone / Antioxidants Language: En Journal: Food Chem Year: 2025 Document type: Article