Photoprotective sulfated mannogalactan from heterotrophic Bacillus velezensis blocks UV-A mediated matrix metalloproteinase expression and nuclear DNA damage in human dermal fibroblast.
J Photochem Photobiol B
; 260: 113022, 2024 Sep 03.
Article
in En
| MEDLINE
| ID: mdl-39288553
ABSTRACT
Prolonged exposure of human dermal fibroblasts (HDF) to ultraviolet (UV) radiation triggers the production of reactive oxygen species by upregulating the expression of matrix metalloproteinases (MMPs), causing type-I collagen degradation and photoaging. A sulfated (1 â 3)/(1 â 4) mannogalactan exopolysaccharide (BVP-2) characterized as [â3)-α-Galp-{(1 â 4)-α-6-O-SO3-Manp}-(1 â 3)-α-6-O-SO3-Galp-(1â] was isolated from seaweed-associated heterotrophic bacterium Bacillus velezensis MTCC13097. Whole genome analysis of B. velezensis MTCC13097 (Accession number JAKYLL000000000) revealed saccharine biosynthetic gene clusters for exopolysaccharide production. BVP-2 administered cells showed noteworthy reduction in mitochondrial superoxide (â¼85 %, p < 0.05) and ROS production (62 %) than those exhibited by UV-A irradiated HDF cells. Oxidative imbalance in HDF cells (after UV-A exposure) was recovered with BVP-2 treatment by significantly downregulating nitric oxide (NO) production (98.6 µM/mL, 1.9-fold) and DNA damage (â67 %) in comparison with UV-A induced cells (191.8 µM/mL and 98.7 %, respectively). UV-irradiated HDF cells showed a â¼30-50 % downregulation in the expression of MMPs (1, 2, and 9) following treatment with BVP-2. Considerable amount of sulfation (18 %) along with (1 â 3)/(1 â 4) glycosidic linkages in BVP-2 could be pivotal factors for down-regulation of the intracellular MMP-1, which was further supported by molecular docking and structure-activity studies. The (1 â 3)/(1 â 4)-linked bacterial exopolysaccharide (BVP-2) might be used as prospective natural lead to attenuate and mitigate UV-A-induced photoaging.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Photochem Photobiol B
Year:
2024
Document type:
Article