Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus.
Nucleic Acids Res
; 12(6): 2619-28, 1984 Mar 26.
Article
in En
| MEDLINE
| ID: mdl-6324124
Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are (formula: see text) with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages lambda and phi X174 and virus SV40.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Deoxyribonucleases, Type II Site-Specific
/
DNA Restriction Enzymes
/
Euryarchaeota
Language:
En
Journal:
Nucleic Acids Res
Year:
1984
Document type:
Article