Use of site-directed antibodies to probe the topography of the alpha 2 subunit of voltage-gated Ca2+ channels.
FEBS Lett
; 364(2): 129-33, 1995 May 08.
Article
in En
| MEDLINE
| ID: mdl-7750557
ABSTRACT
Polyclonal antibodies were raised against peptides corresponding to residues 1-15, 469-483 and 933-951 of the rabbit skeletal muscle L-type calcium channel alpha 2/delta primary translation product, for use as topological probes. Immunocytochemical comparison of the abilities of the antibodies to bind to the alpha 2 and delta subunits in intact and detergent-permeabilised rat dorsal root ganglion cells enabled the membrane orientation of these regions to be established. The resultant data indicate that the regions containing residues 1-15 and 469-483 of the alpha 2 subunit, and residues 1-17 of the delta subunit, are exposed on the extracellular surface of the membrane, findings consistent with a model that proposes alpha 2 to be entirely extracellular.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Calcium Channels
/
Antibodies
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
1995
Document type:
Article