Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion.
FEMS Microbiol Lett
; 110(3): 299-305, 1993 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-8354463
ABSTRACT
Salmonella typhimurium and enteropathogenic Escherichia coli (EPEC) were found to adhere to the brush border of differentiated human intestinal epithelial Caco-2 cells in culture, whereas Yersinia pseudotuberculosis and Listeria monocytogenes adhered to the periphery of undifferentiated Caco-2 cells. All these enterovirulent strains invaded the Caco-2 cells. Using a heat-killed human Lactobacillus acidophilus (strain LB) which strongly adheres both to undifferentiated and differentiated Caco-2 cells, we have studied inhibition of cell association with and invasion within Caco-2 cells by enterovirulent bacteria. Living and heat-killed Lactobacillus acidophilus strain LB inhibited both cell association and invasion of Caco-2 cells by enterovirulent bacteria in a concentration-dependent manner. The mechanism of inhibition of both adhesion and invasion appears to be due to steric hindrance of human enterocytic pathogen receptors by whole-cell lactobacilli rather than to a specific blockade of receptors.
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Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
Bacterial Adhesion
/
Enterobacteriaceae
/
Intestines
/
Lactobacillus acidophilus
/
Listeria monocytogenes
/
Microvilli
Limits:
Humans
Language:
En
Journal:
FEMS Microbiol Lett
Year:
1993
Document type:
Article