Respiration of amniotic fluid in near-term foetal rabbit.
Eur J Obstet Gynecol Reprod Biol
; 62(2): 247-50, 1995 Oct.
Article
in En
| MEDLINE
| ID: mdl-8582505
ABSTRACT
OBJECTIVE:
To examine the existence of amniotic fluid inhalation in foetal rabbit near term. STUDYDESIGN:
Rabbit red cells labelled with 51 radio-chromium (Cr51-S1 Injectable solution sodium chromate, volumic activity 74 MBq/ml; Cis-BioInternational, France) were injected into the amniotic sac of 24 New Zealand White foetal rabbits (mean gestation 31 days) at day 25 per-laparotomy. At day 26, just prior to caesarean section, human serum albumin labelled with 125 radio-active iode (125I-HSA) (SERALB-125 human serum albumin labelled with radioactive Iode 125, volumic activity 185 kBq/ml, Cis-BioInternational, France) was injected into each amniotic sac. The lungs, digestive tracts, kidneys and liver were excised separately and radioactivity counted in each organ.RESULTS:
On day 26 of gestation, the 51Cr-RC radioactivity rate per gram of tissue in lungs, digestive tract amniotic fluid, liver and kidneys were respectively 1.66 +/- 2.8%, 1.15 +/- 1.6%, 0.015 +/- 0.02% and 0.04 +/- 0.07% of the total amount of radioactivity injected into the amniotic sac at day 25. The lungs' radioactivity was significantly higher than liver (t = 2.94, P < 0.01) or kidneys radioactivity (t = 2.38, P < 0.05). The 125I-HSA injected just prior to caesarean section at day 26 was not found in any foetal organ.CONCLUSIONS:
Lung radioactivity is not related to gasps induced by caesarean section, or to a vascular diffusion since lung radioactivity was significantly higher than liver or kidneys' radioactivity. The results of these series of experiments demonstrate that amniotic fluid inhalation does exist in foetal rabbit near term.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Inhalation
/
Fetus
/
Amniotic Fluid
Limits:
Animals
Language:
En
Journal:
Eur J Obstet Gynecol Reprod Biol
Year:
1995
Document type:
Article