Accumulation of gossypol enantiomers in ovine tissues.
Comp Biochem Physiol B Biochem Mol Biol
; 113(2): 417-20, 1996 Feb.
Article
in En
| MEDLINE
| ID: mdl-8653593
ABSTRACT
Tissue residue levels of gossypol enantiomers in cottonseed-fed and lethally intoxicated lambs were determined by the high-performance liquid chromatography-ultraviolet detection method. Gossypol was derivatized with (+)-2-amino-1-propanol and separated with a reversed-phase C18 column and the elution of analytes was monitored at 254 nm. The highest residue level was found in the liver tissue (318-416 ng total gossypol/mg dry tissue), and the residue of (-)-gossypol was higher than (+)-gossypol in the heart, muscle and spleen. The detection limit was 2 ng, and the detector response of gossypol-amine adducts was linear between 2 and 100 ng enantiomers.
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Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
Poisoning
/
Sheep Diseases
/
Gossypol
Limits:
Animals
Language:
En
Journal:
Comp Biochem Physiol B Biochem Mol Biol
Year:
1996
Document type:
Article