Protein kinase C inhibitor H-7 blocks effects of tumor necrosis factor on bone cells.
Zhongguo Yao Li Xue Bao
; 16(6): 509-11, 1995 Nov.
Article
in En
| MEDLINE
| ID: mdl-8732044
ABSTRACT
AIM:
To study the effects of tumor necrosis factor (TNF) on the neonatal mouse osteoblast-enriched calvarial cells and effects of protein kinase C (PK C) inhibitor, 1-(5-isoquinolinesulfonyl)-2 -methylpiperazine (H-7) on the TNF actions.METHODS:
[3H]TdR uptake by the osteoblasts was used to measure cell proliferation. Cellular alkaline phosphatase (AIP) and tartrate resistant acid phosphatase (trAcP) activities were determined spectrophotometrically.RESULTS:
TNF (1-100 kU . L-1) inhibited both proliferation and expression of AIP activity, but stimulated trAcP activity. These TNF-induced actions were blocked by simultaneous addition of H-7 (5-20 mumol . L-1).CONCLUSION:
TNF has potent effects on the osteoblasts, and the blockade of TNF actions by H-7 suggests that TNF exert its effects through PK C.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
Protein Kinase C
/
Tumor Necrosis Factor-alpha
/
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
/
Enzyme Inhibitors
Limits:
Animals
Language:
En
Journal:
Zhongguo Yao Li Xue Bao
Year:
1995
Document type:
Article