Chloride efflux during the progesterone-initiated human sperm acrosome reaction is inhibited by lavendustin A, a tyrosine kinase inhibitor.
J Androl
; 17(4): 327-30, 1996.
Article
in En
| MEDLINE
| ID: mdl-8889693
Previous studies showed that progesterone (P) can initiate the mammalian sperm acrosome reaction (AR) in vitro and that a sperm GABAA-like receptor/Cl- channel is involved in an essential Cl- efflux mediated by P during the AR. Here, we show that lavendustin A, a potent, specific inhibitor of tyrosine kinase activity, strongly inhibits the P-initiated human AR and the essential P-mediated Cl- efflux. Lavendustin B, a weak tyrosine kinase inhibitor, had no significant effect. These results suggest that, as part of AR initiation, P mediates tyrosine phosphorylation of the sperm GABAA- like receptor/Cl- channel.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenols
/
Progesterone
/
Acrosome
/
Chloride Channels
/
Enzyme Inhibitors
Limits:
Humans
/
Male
Language:
En
Journal:
J Androl
Year:
1996
Document type:
Article