Gamma delta T lymphocytes regulate the induction and maintenance of oral tolerance.
J Immunol
; 158(8): 3610-8, 1997 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-9103422
ABSTRACT
Oral administration of Ag, over a period of several days, induces a state of tolerance that is associated with activation of CD8+ T cells that can transfer unresponsiveness to naive syngeneic hosts. We previously demonstrated that these T cells are not CTL precursors and that they inhibit responses by CD8+ CTL, as well as Ab and CD4+ T cell responses. Activation of noncytolytic, CD8+ suppressor T cells by oral Ag is a process that is not understood. In these studies, we asked whether depletion of the gamma delta T cells altered induction of oral tolerance. Injection of the anti-delta-chain Ab (GL3) down-modulated the expression of gamma delta TCR and inhibited the induction of oral tolerance to OVA, as measured by Ab, CD4+, and CD8+ T cell responses. GL3 did not activate IL-2 secretion that could be detected in the serum, nor did it induce IL-2R expression by intraepithelial lymphocytes, suggesting that GL3 inhibited the function of gamma delta T cells rather than activating them. This interpretation is supported by our observation that oral administration of Ag did not induce tolerance in TCR-delta knockout mice. These data suggest that gamma delta T cells play a critical, active role in tolerance induced by orally administered Ag.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Receptors, Antigen, T-Cell, gamma-delta
/
Mouth
/
Antigens
Limits:
Animals
Language:
En
Journal:
J Immunol
Year:
1997
Document type:
Article