Evidence for a localization signal in the 3'untranslated region of myosin heavy chain messenger RNA.
Cell Biol Int
; 21(4): 243-8, 1997 Apr.
Article
in En
| MEDLINE
| ID: mdl-9204538
ABSTRACT
Localization signals in the 3'untranslated region (3'UTR) of myosin heavy chain mRNA were investigated using hybrid gene constructs. In myoblasts transfected with constructs containing either both coding sequences and 3'UTR of the rabbit beta-globin gene or the beta-globin coding sequences alone in situ hybridization showed that globin transcripts were distributed throughout the cytoplasm with no localization. In contrast, in myoblasts transfected with beta-globin coding sequences linked to the myosin heavy chain 3'UTR there was strong perinuclear localization of the hybrid mRNA; this was maintained in myotubes. We conclude that myosin heavy chain 3'UTR contains a localization signal.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
RNA, Messenger
/
Myosin Heavy Chains
Limits:
Animals
Language:
En
Journal:
Cell Biol Int
Year:
1997
Document type:
Article