Context dependent RNA-RNA recognition in a three-dimensional model of the 16S rRNA core.
Bioorg Med Chem
; 5(6): 1021-35, 1997 Jun.
Article
in En
| MEDLINE
| ID: mdl-9222495
ABSTRACT
A 3-D model of the core of the 16S rRNA of Escherichia coli containing 328 residues has been built in the protein map derived from neutron scattering data with the help of all the available phylogenetic, biochemical, and cross-linking data. The three pseudoknots of the 16S-core cluster, through the arrangement of complex three-, four- and five-way junctions, around the neck and at the subunit interface. The roles in assembly, initiation or elongation of the three pseudoknots in ribosomal dynamics are emphasized. The 530-loop, localized on the periphery of the 30S particle, could be built with and without a pseudoknot independently of the state of the particle. The pseudoknot of the central domain controls the dynamics of an helix connected to the subunit interface which could trigger some mechanism during translation. The process of the model construction is compatible with a folding scenario in which the 5'-terminal pseudoknot controls the assembly of the central junction and the subsequent folding of the 3'-major domain. The modelling, together with the phylogenetic analysis and the experimental data, point to several potential RNA-RNA contacts which depend on the structural and sequence context in which they occur.
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Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
RNA
/
RNA, Ribosomal, 16S
/
Models, Molecular
/
Nucleic Acid Conformation
Language:
En
Journal:
Bioorg Med Chem
Year:
1997
Document type:
Article