Regulation of a plant plasma membrane sucrose transporter by phosphorylation.
FEBS Lett
; 424(3): 165-8, 1998 Mar 13.
Article
in En
| MEDLINE
| ID: mdl-9539143
ABSTRACT
The protein phosphatase inhibitor okadaic acid (OA) either provided directly to sugar beet (Beta vulgaris L.) leaf discs or infiltrated in the leaf blade rapidly inhibited sucrose uptake. Methyl okadaic acid, a biologically inactive analogue of OA, had only a marginal effect on uptake. OA inhibited proton-motive force-driven uptake of sucrose into plasma membrane vesicles, without affecting their proton permeability. OA did not significantly affect the amount of sucrose transporters present in the vesicles, as estimated by ELISA with specific antibodies. It is concluded that OA directly inhibits the activity of a H+-sucrose cotransporter of the plant plasma membrane, likely by maintaining it in a phosphorylated form.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Transport Proteins
/
Plant Proteins
/
Sucrose
/
Carrier Proteins
/
Cell Membrane
/
Okadaic Acid
/
Chenopodiaceae
Language:
En
Journal:
FEBS Lett
Year:
1998
Document type:
Article