Magic angle spinning NMR of the protonated retinylidene Schiff base nitrogen in rhodopsin: expression of 15N-lysine- and 13C-glycine-labeled opsin in a stable cell line.

Eilers, M; Reeves, P J; Ying, W; Khorana, H G; Smith, S O

Eilers, M; Reeves, P J; Ying, W; Khorana, H G; Smith, S O.

Affiliation

Eilers M; Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA.

Proc Natl Acad Sci U S A ; 96(2): 487-92, 1999 Jan 19.

Article in En | MEDLINE | ID: mdl-9892660

Subject(s)

Rhodopsin/chemistry; Rod Opsins/metabolism; Schiff Bases/chemistry; Amino Acid Sequence; Animals; Carbon Isotopes/analysis; Cattle; Cell Line; Humans; Liposomes/metabolism; Lysine/chemistry; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Molecular Structure; Nitrogen Isotopes/analysis; Protein Structure, Secondary; Retinaldehyde/metabolism; Spectrophotometry; Water/chemistry

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rhodopsin / Schiff Bases / Rod Opsins Limits: Animals / Humans Language: En Journal: Proc Natl Acad Sci U S A Year: 1999 Document type: Article

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