Could kDNA-PCR in peripheral blood replace the examination of bone marrow for the diagnosis of visceral leishmaniasis?
J. parasitol. res
; : [1084353], Aug. 2016. tab
Article
em En
| SES-SP, SESSP-IIERPROD, SES-SP
| ID: biblio-1021497
Biblioteca responsável:
BR31.1
Localização: BR31.1; 2016_P-013
ABSTRACT
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. Based on the GS, 38 samples from 32 patients were grouped Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases. In order to evaluate the parasitological and molecular techniques in PB, the samples were examined. From Group 1, PB kDNA-PCR was positive in 20 samples and in 19 of 20 in BM kDNA-PCR examination. However, the parasitological examination of buffy coat was insensitive, being able to detect only 4 cases from Group 1. All samples from Group 2 were negative. We concluded that, for the diagnosis of visceral leishmaniasis, the parasitological examination of peripheral blood was not useful; however, molecular diagnosis by kDNA-PCR, performed in peripheral blood, could be useful to replace the parasitological examination of bone marrow
Texto completo:
1
Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IIERPROD
Assunto principal:
Exame de Medula Óssea
/
Reação em Cadeia da Polimerase
/
DNA de Cinetoplasto
/
Leishmaniose Visceral
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J. parasitol. res
Ano de publicação:
2016
Tipo de documento:
Article