Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats
Braz. j. med. biol. res
; 53(1): e8389, Jan. 2020. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1055479
Biblioteca responsável:
BR1.1
ABSTRACT
Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy metabolism and oxidative stress in T1DM Wistar rats was investigated. Evaluation of the acute effects of this pigment was performed by incubation of isolated hepatocytes with chlorophyll-a and the chronic effects were evaluated by oral treatment with chlorophyll-based extract, with post-analysis of the intact liver by in situ perfusion. In both experimental protocols, chlorophyll-a decreased hepatic glucose release and glycogenolysis rate and stimulated the glycolytic pathway in DM/PDT. In addition, there was a reduction in hepatic oxidative stress, noticeable by decreased lipoperoxidation, reactive oxygen species, and carbonylated proteins in livers of chlorophyll-treated T1DM rats. These are indicators of the potential capacity of chlorophyll-a in improving the status of the diabetic liver.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
LILACS
Assunto principal:
Clorofila
/
Fármacos Fotossensibilizantes
/
Estresse Oxidativo
/
Diabetes Mellitus Experimental
/
Glicólise
/
Fígado
Tipo de estudo:
Guia de prática clínica
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Ano de publicação:
2020
Tipo de documento:
Artigo
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