Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates
Parasitology Research
; 108(1): 123-135, Sept 18, 2010.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1065154
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
Schistosomiasis affects more than 200 million people worldwide; another 600 million are at risk ofinfection. The schistosomulum stage is believed to be the target of protective immunity in the attenuated cercaria vaccine model. In an attempt to identify genes upregulated in the schistosomulum stage in relation to cercaria, we explored the Schistosoma mansoni transcriptome by looking at the relative frequency of reads in EST libraries from both stages. The 400 genes potentially up-regulated in schistosomula were analyzed as to their Gene Ontology categorization, and we have focused on those encoding-predicted proteins with no similarity to proteins of other organisms, assuming they could be parasite-specific proteins important for survival in the host. Up-regulation in schistosomulum relative to cercaria was validated with real-time reverse transcription polymerase chain reaction (RT-PCR) for five out of nine selected genes (56%). We tested their protective potential in mice through immunization with DNA vaccines followed by a parasite challenge. Worm burden reductions of 1617% were observed for one of them, indicating its protective potential. Our results demonstrate the value and caveats of using stage-associated frequency of ESTs as na indication of differential expression coupled to DNA vaccine screening in the identification of novel proteins to be further investigated as potential vaccine candidates.
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Coleções:
06-national
/
BR
Contexto em Saúde:
2_ODS3
/
3_ND
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Schistosoma mansoni
/
Imunização
/
Produção de Produtos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Parasitology Research
Ano de publicação:
2010
Tipo de documento:
Article