The therapeutic potential of recombinant BCG expressing the antigen S1PT in the intravesical treatment of bladder cancer
Urol. oncol
; 28(5): 520-525, sept.-oct. 2010.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1068322
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
Bacillus Calmette-Guerin (BCG) continues to be employed as the most effective immunotherapy against superficial bladder cancer. We have developed an rBCG-S1PT strain that induces a stronger cellular immune response than BCG. This preclinical study was designed to test the potential of rBCG-S1PT as an immunotherapeutic agent for intravesical bladder cancer therapy.A tumor was induced in C57BL/6 mice after chemical cauterization of the bladder and inoculation of the tumor cell line MB49. Next, mice were treated by intravesical instillation with BCG, rBCG-S1PT, or PBS once a week for 4 weeks. After 35 days, the bladders were removed and weighed, Th1 (IL-2, IL-12, INOS, INF-ã, TNF-á), and Th2 (IL-5, IL-6, IL-10, TGF-â) cytokine mRNA responses in individual mice bladders were measured by quantitative real time PCR, and the viability of MB49 cells in 18-hour coculture with splenocytes from treated mice was assessed. In an equivalent experiment, animals were observed for 60 days to quantify their survival.Both BCG and rBCG-S1PT immunotherapy resulted in bladder weight reduction, and rBCG-S1PT increased survival time compared with the control group. There were increases in TNF-á in the BCG treated group, as well as increases in TNF-á and IL-10 mRNA in the rBCG-S1PT group. The viability of MB49 cells cocultured with splenocytes from rBCG-S1PT-treated mice was lower than in both the BCG and control groups.rBCG-S1PT therapy improved outcomes and lengthened survival times. These results indicate that rBCG could serve as a useful substitute for wild-type BCG.
Palavras-chave
Buscar no Google
Coleções:
06-national
/
BR
Contexto em Saúde:
3_ND
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Neoplasias da Bexiga Urinária
/
Vacina BCG
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Urol. oncol
Ano de publicação:
2010
Tipo de documento:
Article