Cell surface proteins play an important role in probiotic activities of Lactobacillus reuteri
Nutrire Rev. Soc. Bras. Aliment. Nutr
; 41: 1-10, Dec. 2016. tab, ilus
Article
em En
| LILACS
| ID: biblio-880608
Biblioteca responsável:
BR1208.1
ABSTRACT
BACKGROUND:
Eight Lactobacillus reuteri strains, previously isolated from breast-fed human infant feces, were selected to assess the potential contribution of their surface proteins in probiotic activity. These strains were treated with 5 M LiCl to remove their surface proteins, and their tolerance to simulated stomach-duodenum passage, cell surface characteristics, auto aggregation, adhesion, and inhibition of pathogen adhesion to Caco-2 cells were compared with untreated strains.RESULTS:
The survival rates, auto aggregation, and adhesion abilities of the LiCl-treated L. Reuteri strains decreased significantly (p< 0.05) compared to that of the untreated cells. The inhibition ability of selected L. reuteri strains, untreated or LiCl treated, against adherence of Escherichia coli 25922 and Salmonella typh iNCDC113 to Caco-2 was evaluated in vitro with L. reuteri ATCC55730 strain as a positive control. Among the selected eight strains of L.reuteri, LR6 showed maximum inhibition against the E. Coli ATCC25922 and S. typhiNCDC113. After treatment with 5 M LiCl to remove surface protein, the inhibition activities of the lactobacilli against pathogens decreased significantly (p< 0.05). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated thatLR6 strains had several bands with molecular weight ranging from 10 to 100 KDa, and their characterization and functions need to be confirmed.CONCLUSIONS:
The results revealed that the cell surface proteins of L. reuteri play an important role in their survivability, adhesion, and competitive exclusion of pathogen to epithelial cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Probióticos
/
Limosilactobacillus reuteri
/
Proteínas de Membrana
Idioma:
En
Revista:
Nutrire (Impresso)
/
Nutrire (Online)
/
Nutrire Rev. Soc. Bras. Aliment. Nutr
Ano de publicação:
2016
Tipo de documento:
Article