Effects of the application of the amniotic membrane in the healing process of skin wounds in rats
Acta cir. bras
; 33(2): 144-155, Feb. 2018. graf
Article
em En
| LILACS
| ID: biblio-886256
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats.Methods:
Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18) group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated.Results:
The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05).Conclusion:
Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Pele
/
Cicatrização
/
Curativos Biológicos
/
Colágeno
/
Âmnio
Tipo de estudo:
Clinical_trials
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Acta cir. bras
Ano de publicação:
2018
Tipo de documento:
Article