Search for DQ2 5 and DQ8 alleles using a lower cost technique in patients with type 1 diabetes and celiac disease in a population of southern Brazil
Arch. endocrinol. metab. (Online)
; 61(6): 550-555, Dec. 2017. tab
Article
em En
| LILACS
| ID: biblio-887614
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Objective To evaluate the frequency of DQ2.5 and DQ8 alleles using the Tag-single-nucleotide polymorphism (Tag-SNP) technique in individuals with type 1 diabetes mellitus (T1DM) and celiac disease (CD) in southern Brazil. Materials and methods In a prospective design, we performed the search for DQA1*0501 and DQB1*0201 alleles for DQ2.5 and DQB1*0302 for DQ8 through Real-Time Polymerase Chain Reaction (RT-PCR) technique, using TaqMan Genotyping Assays (Applied Biosystems, USA). The diagnosis of CD was established by duodenal biopsy and genotypic determination performed by StepOne Software v2.3. Allelic and genotypic frequencies were compared between groups using Chi-square and Fisher's exact tests and the multiple comparisons using Finner's adjustment. Results Three hundred and sixty two patients with a median age of 14 years were divided into 3 groups T1DM without CD (264); T1DM with CD (32) and CD without T1DM (66). In 97% of individuals with T1DM and CD and 76% of individuals with CD without T1DM, respectively, the alleles DQ2.5 and/or DQ8 were identified (p < 0.001). DQ2.5 was more common in individuals with CD (p = 0.004) and DQ8 was more common in individuals with type 1 diabetes (p = 0.008). Conclusions The evaluation of the alleles for DQ2.5 and DQ8 by Tag-SNP technique showed a high negative predictive value among those with T1DM, similar to that described by the conventional technique. The high frequency of DQ8 alleles in individuals with T1DM did not allow differentiating those at higher risk of developing T1DM.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Aperfeicoar_gestao_SUS
Contexto em Saúde:
1_ASSA2030
Base de dados:
LILACS
Assunto principal:
Doença Celíaca
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Predisposição Genética para Doença
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Diabetes Mellitus Tipo 1
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Frequência do Gene
Tipo de estudo:
Etiology_studies
/
Health_economic_evaluation
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Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Arch Endocrinol Metab
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Arch. endocrinol. metab. (Online)
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Archives of endocrinology and metabolism (Impresso)
/
Archives of endocrinology and metabolism (Online)
Ano de publicação:
2017
Tipo de documento:
Article