Mycobacterium tuberculosis promotes genomic instability in macrophages
Mem. Inst. Oswaldo Cruz
; 113(3): 161-166, Mar. 2018. tab, graf
Article
em En
| LILACS
| ID: biblio-894903
Biblioteca responsável:
BR1.1
ABSTRACT
BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
LILACS
Assunto principal:
Hibridização in Situ Fluorescente
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Instabilidade Genômica
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Mycobacterium tuberculosis
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2018
Tipo de documento:
Article