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Experimental allergic airway inflammation impacts gut homeostasis in mice
Heliyon, v. 9, n. 6, e16429, jun. 2023
Article em En | SES-SP, SESSP-IBPROD, SES-SP | ID: bud-4971
Biblioteca responsável: BR78.1
ABSTRACT
Background

Aims:

Epidemiological data show that there is an important relationship between respiratory and intestinal diseases. To improve our understanding on the interconnectedness between the lung and intestinal mucosa and the overlap between respiratory and intestinal diseases, our aim was to investigate the influence of ovalbumin (OVA)-induced allergic airway inflammation on gut homeostasis. Methods A/J mice were sensitized and challenged with OVA. The animals were euthanized 24 h after the last challenge, lung inflammation was determined by evaluating cells in Bronchoalveolar lavage fluid, serum anti-OVA IgG titers and colon morphology, inflammation and integrity of the intestinal mucosa were investigated. IL-4 and IL-13 levels and myeloperoxidase activity were determined in the colon samples. The expression of genes involved in inflammation and mucin production at the gut mucosa was also evaluated. Results OVA challenge resulted not only in lung inflammation but also in macroscopic alterations in the gut such as colon shortening, increased myeloperoxidase activity and loss of integrity in the colonic mucosal. Neutral mucin intensity was lower in the OVA group, which was followed by down-regulation of transcription of ATOH1 and up-regulation of TJP1 and MUC2. In addition, the OVA group had higher levels of IL-13 and IL-4 in the colon. Ova-specific IgG1 and OVA-specific IgG2a titers were higher in the serum of the OVA group than in controls. Conclusions Our data using the OVA experimental model suggested that challenges in the respiratory system may result not only in allergic airway inflammation but also in the loss of gut homeostasis.
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Texto completo: 1 Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBPROD Idioma: En Revista: Heliyon Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBPROD Idioma: En Revista: Heliyon Ano de publicação: 2023 Tipo de documento: Article