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Serum proteome in acute myocardial infarction

Cubedo, Judit; Padró, Teresa; García-Moll, Xavier; Cinca, Juan; Pintó, Xavier; Badimon, Lina.
Clín. investig. arterioscler. (Ed. impr.); 23(4): 147-154, jul.-ago. 2011. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-92898

Introduction:

Acute myocardial infarction (AMI) is one of the major causes of mortality and morbidity worldwide. Despite the efforts being made, there is a lack of early markers for the prevention, diagnosis and treatment of ischemic syndromes. Proteomic expression profiling technologies are a highly important tool for research into new serum biomarkers for the diagnosis and prognosis of acute coronary syndromes.

Methods:

Serum samples were sub-fractionated with different methods for the depletion of high-abundance proteins. The low-abundance fraction was analyzed by two-dimensional electrophoresis(2-DE), followed by protein identification with mass-spectrometry (MALDI-TOF).The proteomic profiles of serum samples from AMI patients and controls were analyzed and compared.

Results:

Through depletion of six high-abundance proteins in 2-DE analysis of serum samples,569 spots were detected, of which 131 spots were only detected in the AMI group and 27 were only detected in controls. The comparative analysis between AMI-patients and controls revealed a group of differential protein spots involved in seven different biological functions. The main changes were found in proteins involved in the immune system and lipid metabolism.

Conclusions:

In this study, by using a 2-DE differential approach, we developed a highly reproducible methodology for the analysis of coordinated changes in serum proteome patterns that occur within the first 6 hours after the onset of an AMI (AU)
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