Serum proteome in acute myocardial infarction
Clín. investig. arterioscler. (Ed. impr.)
; 23(4): 147-154, jul.-ago. 2011. tab, ilus
Artigo
em Espanhol
| IBECS
| ID: ibc-92898
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Introduction:
Acute myocardial infarction (AMI) is one of the major causes of mortality and morbidity worldwide. Despite the efforts being made, there is a lack of early markers for the prevention, diagnosis and treatment of ischemic syndromes. Proteomic expression profiling technologies are a highly important tool for research into new serum biomarkers for the diagnosis and prognosis of acute coronary syndromes.Methods:
Serum samples were sub-fractionated with different methods for the depletion of high-abundance proteins. The low-abundance fraction was analyzed by two-dimensional electrophoresis(2-DE), followed by protein identification with mass-spectrometry (MALDI-TOF).The proteomic profiles of serum samples from AMI patients and controls were analyzed and compared.Results:
Through depletion of six high-abundance proteins in 2-DE analysis of serum samples,569 spots were detected, of which 131 spots were only detected in the AMI group and 27 were only detected in controls. The comparative analysis between AMI-patients and controls revealed a group of differential protein spots involved in seven different biological functions. The main changes were found in proteins involved in the immune system and lipid metabolism.Conclusions:
In this study, by using a 2-DE differential approach, we developed a highly reproducible methodology for the analysis of coordinated changes in serum proteome patterns that occur within the first 6 hours after the onset of an AMI (AU)ABSTRACT
Introducción El infarto agudo de miocardio (IAM) es una de las mayores causas de mortalidad y morbilidad en el mundo. A pesar de todos los esfuerzos hay una falta de marcadores para la prevención, el diagnóstico y el tratamiento de la fase temprana de los síndromes isquémicos. Las técnicas proteómicas son una herramienta muy importante para la búsqueda de nuevos biomarcadores para el diagnóstico y el pronóstico de los síndromes coronarios agudos. Métodos Las muestras de suero se sub-fraccionaron mediante diferentes métodos para eliminar las proteínas mayoritarias. La fracción de proteínas de menor abundancia se analizó mediante electroforesis bidimensional (2-DE), seguida de la identificación de proteínas mediante espectrometría de masas (MALDI-TOF). Se analizó y comparó el patrón proteómico de los pacientes IAM y el grupo control. Resultados Mediante la eliminación de las seis proteínas mayoritarias en el análisis por 2-DE se detectaron un total de 569 spots, de los cuales 131 sólo se detectaron en el grupo infarto y 27 sólo en el grupo control. El análisis comparativo entre los pacientes IAM y los controles reveló un grupo de spots proteicos con un patrón de (..) (AU)
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Coleções:
Bases de dados nacionais
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Espanha
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
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6_ODS3_enfermedades_notrasmisibles
Base de dados:
IBECS
Assunto principal:
Proteoma
/
Infarto do Miocárdio
Limite:
Humanos
Idioma:
Espanhol
Revista:
Clín. investig. arterioscler. (Ed. impr.)
Ano de publicação:
2011
Tipo de documento:
Artigo
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