Inhibition of enteropathogenic Escherichia coli adherence to Hela cells by immune rabbit sera
Braz. j. med. biol. res
; 25(8): 809-12, 1992. tab
Artigo
em Inglês
| LILACS
| ID: lil-113573
Biblioteca responsável:
BR26.1
ABSTRACT
We have studied the effect of immune rabbit sera on the localized (LA) and diffuse (DA) adherence to Hela cells of 10 enteropathogenic Escherichia coli (EPEC) strains belonging to serogroups 055, 086, 0111, 0119, and 0142. Anti-La1 serum, obtained by rabbit immunization with an E. coli strain harboring a cloned DNA fragment from an EPEC LA plasmid, strongly inhibited the adherence of all serogroups but one (0142). Similar results were obtained with anti-LA2 serum, which is anti-0111 serum absorbed with a non-adherence 0111H-EPEC strain. In contrast, non-absorbed anti-055 and anti-0111 sera showed an inhibitory effect mainly on the adherence of homologous strains. Except for one experiment diffuse adherence was not inhibited by any antiserum used. The inhibitory effect of immune sera on localized adhere3nce does not seem to be correlated with plasmid curing sinceadherence plasmid pMS49 proved to be stable a after treatment with anti-055 and anti-0111 sera. The cross-inhibition of adherence by anti-LA sera suggests that localized adherence-related adhesions of the 0.55, 0.86, 0111, and 0119 strains share similar antigens
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Contexto em Saúde:
Doenças Neglicenciadas
Base de dados:
LILACS
Assunto principal:
Plasmídeos
/
Aderência Bacteriana
/
Diarreia Infantil
/
Escherichia coli
/
Soros Imunes
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Ano de publicação:
1992
Tipo de documento:
Artigo
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