Fluoride and phosphatidylserine induced inhibition of cytosolic insulin-degrading activity
Acta physiol. pharmacol. ther. latinoam
; 42(3): 183-96, 1992. tab, graf
Article
em En
| LILACS
| ID: lil-134661
Biblioteca responsável:
BR1.1
RESUMO
Cytosol (C) (100,000 x g/60 min, supernatant) from liver, brain and testis (Wistar male rats) are shown to contain insulin degrading activity (C-IDA). The regulation of C-IDA in these fractions by ligands that activate G protein and PKC were examined C-IDA from liver, brain and testis was inhibited 76%; 64% and 50% by 50 mM F- respectively. Chromatography of C fraction from liver on Sephadex G-50 in presence of 1 M (NH4)2SO4 and 20% (v/v) glycerol (experimental condition to remove guanine nucleotides from G proteins) decreased in about 3-fold aluminum fluoride effect on C-IDA. Mg++ (from 5mM to 10 mM) enhanced fluoride effects by inhibiting fully C-IDA. Phosphatidylserine in presence of ATP completely inhibited C-IDA; this inhibition was 31.3% mediated by a phosphorylation reaction. It is concluded that cytosol from different tissues contain proteins capable to associate ligands as aluminum fluoride and PS to regulate C-IDA. It is proposed a mechanism of protein-protein interaction to modulate C-IDA
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Fosfatidilserinas
/
Técnicas In Vitro
/
Citosol
/
Fluoretos
/
Insulina
Limite:
Animals
Idioma:
En
Revista:
APPTLA
/
Acta Physiol Pharmacol Ther Latinoam
/
Acta physiol. pharmacol. ther. latinoam
/
Acta physiologica pharmacologica et therapeutica latinoamericana
Ano de publicação:
1992
Tipo de documento:
Article